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CASE REPORT
CytoJournal 2016,  13:29

An unexpected diagnosis of ectopic liver diagnosed by fine needle aspiration


1 Department of Pathology, University of Utah, Salt Lake City, Utah, USA
2 Department of Gastroenterology and Hepatology, University of Utah, Salt Lake City, Utah, USA

Date of Submission02-May-2016
Date of Acceptance18-Jun-2016
Date of Web Publication20-Dec-2016

Correspondence Address:
Rachel E Factor
Department of Pathology, University of Utah, Salt Lake City, Utah
USA
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1742-6413.196239

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 » Abstract 

The differential diagnosis of perigastric masses is broad, ranging from benign to malignant entities. Among the benign entities, accessory liver lobes and ectopic liver are unusual and often incidentally discovered. Here, we report a patient with malignant melanoma who was clinically suspected to have a perigastric metastasis or a gastrointestinal stromal tumor but was ultimately diagnosed by fine needle aspiration (FNA) to have benign ectopic liver. A 47-year-old male was diagnosed with malignant melanoma of the scalp in May 2015 at a tertiary care hospital. He was found to have a 2.6 cm enhancing mass adjacent to the fundus of the stomach and below the diaphragm by computed tomography imaging. To exclude metastasis, the patient was referred to endoscopy, and an endoscopic ultrasound-guided FNA was performed with rapid on-site evaluation (ROSE) by a cytopathologist. A relatively new FNA needle (Shark Core) was used, which produced useful core biopsy material. Cytopathology demonstrated flat sheets, single cells, and small clusters of polygonal cells. There was abundant granular cytoplasm, often containing pigment. Cells lacked pleomorphism. The smear findings appeared consistent with hepatocytes. The cell block demonstrated small core fragments of hepatic parenchyma with portal tracts. Immunohistochemistry for arginase-1 confirmed that this was hepatic tissue. ROSE was useful for communicating with the endoscopist that the mass was both far from, and not connected to, the liver. This is the first documented account of perigastric ectopic liver diagnosed by FNA. This entity should be considered in the differential of perigastric masses.


Keywords: Ectopic liver, fine needle aspiration, Shark Core needle


How to cite this article:
Barazza G, Adler DG, Factor RE. An unexpected diagnosis of ectopic liver diagnosed by fine needle aspiration. CytoJournal 2016;13:29

How to cite this URL:
Barazza G, Adler DG, Factor RE. An unexpected diagnosis of ectopic liver diagnosed by fine needle aspiration. CytoJournal [serial online] 2016 [cited 2017 Mar 30];13:29. Available from: http://www.cytojournal.com/text.asp?2016/13/1/29/196239



 » Introduction Top


Accessory and ectopic liver lobes have a low incidence and are usually identified during imaging studies or autopsy. [1] These lesions result from embryologic heteroplasia which may be congenital or acquired. [2] They are classified as accessory liver lobes when connected to the liver by a stalk and as ectopic liver tissue when there is no connection. Reports of ectopic liver are rare although this entity has been reported in the thorax, lung, gallbladder, pancreas, umbilicus, adrenals, pylorus, and inferior vena cava. [1],[3],[4] In the abdomen, patients rarely manifest physical complaints, unless complications occur, such as torsion, bleeding, or obstruction. [2],[5],[6]


 » Case Report Top


A 47-year-old male was diagnosed with malignant melanoma of the scalp in May 2015 at our tertiary care hospital. In reviewing treatment options, he underwent imaging surveillance, including an abdominal computed tomography. This showed a 2.6 cm enhancing mass adjacent to the fundus of the stomach and immediately below the diaphragm [Figure 1]a and b. The radiology differential included a gastrointestinal stromal tumor (GIST), splenule, and metastatic melanoma. The patient had opted to receive biochemotherapy for his primary melanoma, and a biopsy of the perigastric mass was needed to exclude a metastasis. The patient was referred to endoscopy.
Figure 1: (a and b) Computerized tomography scans of the abdomen showing a subdiaphragmatic mass (shown with arrow). (c) Endoscopic ultrasound image showing the needle within the mass. (d) Clusters of polygonal cells with distinct borders, smooth nuclear contours, and abundant cytoplasm containing pigment (Diff-Quik, ×40). (e) Cell block showing hepatic tissue with portal tracts (H and E, ×20). (f) Hepatic tissue confirmed by arginase-1 immunostain

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Endoscopic ultrasound (EUS) identified a 2.5 cm oval mass in the perigastric space below the diaphragm, without definite involvement of the stomach. These images were suggestive of a lesion arising independently from the gastric wall. Five passes were performed with a 22-gauge Shark Core needle (Medtronic, Dublin, Ireland) to maximize tissue acquisition [Figure 1]c. Rapid on-site evaluation (ROSE) was performed by a cytopathologist. After reviewing the first pass, the cytopathologist communicated with the endoscopist to ask if the needle had passed through the liver. The endoscopist confirmed that the needle was not in or near the liver and that it had been passed through the stomach to obtain the biopsy. Small tissue fragments were touched on the slide, and those which did not smear were placed in formalin for the cell block preparation.

Diff-Quik smears of the second fine needle aspiration (FNA) demonstrated flat sheets, single cells, and small clusters of polygonal cells with distinct cell borders, round to ovoid centrally placed nuclei with some size variation, but smooth nuclear contours, open chromatin, and no pleomorphism. Occasionally, prominent nucleoli were identified. Cells had abundant granular cytoplasm which contained some pigments [Figure 1]d. The smear findings appeared consistent with hepatocytes. The cell block confirmed this, demonstrating small core fragments of hepatic parenchyma with portal tracts. Immunohistochemistry for arginase-1 confirmed that this was hepatic tissue [Figure 1]e and f and a reticulin stain demonstrated normal hepatic plates (figure not shown). The cytologic and histologic findings, in conjunction with the imaging, were consistent with ectopic liver.


 » Discussion Top


The differential diagnosis of an incidentally discovered perigastric mass is broad and includes both benign and neoplastic entities. Benign primary lesions and neoplasms include, but are not limited to, leiomyoma, schwannoma, neurofibroma, GIST, lymphoma, and carcinoid. Metastases may also present as an incidental lesion. In our case, the top clinical differential included GIST or metastatic melanoma.

Although it is a rare entity, it is important to realize that ectopic liver is in the differential of abdominal and perigastric lesions. In our case, the FNA Diff-Quik smear demonstrated cytology that appeared consistent with benign hepatocytes during ROSE, which raised the possibility of ectopic liver. However, confirmation that this was not one of the other entities in the differential was critical, especially since they can have overlapping cytologic features. Epithelioid GISTs, for instance, may be admixed with or entirely lack spindle cells. They usually demonstrate intermediate-sized cells with round to occasionally irregular nuclei, intranuclear inclusions, some binucleations, and scant to moderate clear to granular cytoplasm, which tends to get pulled away from the cell clusters in wispy strands. [7] Malignant melanoma has variable cytologic findings. Cells may be spindled, epithelioid, or mixed, and while they may demonstrate frank dysplasia, with large nuclei, prominent nucleoli, and abundant cytoplasm, they can also look bland, and like hepatocytes and epithelioid GISTs, binucleation, intracytoplasmic vacuoles, and intracytoplasmic pigment are occasional findings. [8]

ROSE, with active intraprocedural communication between the cytologist and the endoscopist, was extremely helpful in this case to rule out the possibility of liver contamination. When cells that appeared to be hepatocytes were noted on the first few passes, there was an immediate discussion with the endoscopist about the placement of the needle. It was confirmed the needle passed through the stomach to obtain the biopsy, and was within the perigastric mass, far from the liver.

Confirmation that the lesion consisted of benign hepatocytes was ultimately facilitated by use of a new EUS needle (Shark Core) designed to give either generous cytology samples by touch prep or core biopsy material for the cell block. Tissue architecture was available, and there was abundant material to perform immunohistochemistry. In addition to confirming the presence of hepatic tissue, it was also useful to exclude the possibility of hepatocellular carcinoma, which has been reported in ectopic liver. [9],[10],[11],[12] This needle is new to the US market. One pilot study on this device currently in press was performed at our institution and showed that the device performs very well when compared to a standard EUS-FNA needle in terms of yield and reduced number of passes. [13]

In summary, ectopic liver is rare and usually incidentally discovered entity. The cytology from the material biopsied by FNA may have overlapping features with other entities more commonly considered in the differential of perigastric masses. ROSE is helpful to communicate with the endoscopist about placement of the needle. From our literature review, while FNA of presumed ectopic liver within the thorax has been reported, [12] ours is the first report of perigastric ectopic liver diagnosed by FNA.


 » Competing Interests Statement By All Authors Top


All authors declare no competing interests.


 » Authorship Statement By All Authors Top


All authors declare that we qualify for authorship as defined by ICMJE http://www.icmje.org/ethical_1author.html.

DA performed the endoscopy, was involved in the clinical management of the patient, and drafted portions of the manuscript. GB and REF performed both the on site evaluation of the case, and arrived at the final diagnosis. GB performed background research. REF drafted the manuscript. All authors read and approved the final manuscript.


 » Ethics Statement By All Authors Top


As this is a case report without identifiers, our institution does not require approval from the Institutional Review Board (IRB).


 » List Of Abbreviations Top


(In alphabetic order)

EUS - Endoscopic ultrasound

EUS-FNA - Endoscopic ultrasound-guided FNA

FNA - Fine needle aspiration

GIST - Gastrointestinal stromal tumor

IVC - Inferior vena cava

ROSE - Rapid on-site evaluation.

 
 » References Top

1.
Leone N, Saettone S, De Paolis P, Carucci P, Brunello F, De Angelis C, et al. Ectopic livers and related pathology: Report of three cases of benign lesions. Dig Dis Sci 2005;50:1818-22.  Back to cited text no. 1
    
2.
Wang C, Cheng L, Zhang Z, Xie T, Ding H, Deng Q, et al. Accessory lobes of the liver: A report of 3 cases and review of the literature. Intractable Rare Dis Res 2012;1:86-91.  Back to cited text no. 2
    
3.
Park WH, Choi SO, Lee SS, Randolph JG. Ectopic umbilical liver in conjunction with biliary atresia: Uncommon association. J Pediatr Surg 1991;26:219-22.  Back to cited text no. 3
    
4.
Griniatsos J, Riaz AA, Isla AM. Two cases of ectopic liver attached to the gallbladder wall. HPB (Oxford) 2002;4:191-4.  Back to cited text no. 4
    
5.
Glenisson M, Salloum C, Lim C, Lacaze L, Malek A, Enriquez A, et al. Accessory liver lobes: Anatomical description and clinical implications. J Visc Surg 2014;151:451-5.  Back to cited text no. 5
    
6.
Morris MW Jr., Helling TS, Creswell LL, Jordan B, Mitchell ME. Ectopic liver masquerading as a floating intracaval mass. J Vasc Surg 2012;55:1759-61.  Back to cited text no. 6
    
7.
Dong Q, McKee G, Pitman M, Geisinger K, Tambouret R. Epithelioid variant of gastrointestinal stromal tumor: Diagnosis by fine-needle aspiration. Diagn Cytopathol 2003;29:55-60.  Back to cited text no. 7
    
8.
DeMay M. Practical Principles of Cytopathology. Chicago, IL: American Society for Clinical Pathology Press; 2007. p. 159-60.  Back to cited text no. 8
    
9.
Cardona D, Grobmyer S, Crawford JM, Liu C. Hepatocellular carcinoma arising from ectopic liver tissue in the pancreas. Virchows Arch 2007;450:225-9.  Back to cited text no. 9
    
10.
Shigemori M, Kondo M, Azechi H, Inoue F, Tamura J, Kobayashi H, et al. A case of ectopic hepatocellular carcinoma in the jejunum. J Gastroenterol 2006;41:913-8.  Back to cited text no. 10
    
11.
Dettmer M, Itin P, Miny P, Gandhi M, Cathomas G, Willi N. Giant ectopic liver, hepatocellular carcinoma and pachydermia-a rare genetic syndrome? Diagn Pathol 2011;6:75.  Back to cited text no. 11
    
12.
Khalbuss WE, Bajestani S, D'Agostino HJ. Cytomorphology of a solitary left chest wall mass: An unusual presentation from unknown primary hepatocellular carcinoma. Diagn Cytopathol 2007;35:586-9.  Back to cited text no. 12
    
13.
Adler DG, Witt B, Chadwick B, Wells J, Taylor LJ, Dimaio C, et al. Pathologic evaluation of a new EUS needle designed to obtain core tissue samples: A pilot study. Endosc Ultrasound 2016;5:178-83.  Back to cited text no. 13
[PUBMED]  Medknow Journal  

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To ensure the integrity and the highest quality of CytoJournal publications, the review process of this manuscript was conducted under a double blind model (authors are blinded for reviewers and vice versa) through automatic online system.


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