Translate this page into:
Cervical lymphadenopathy with dual pathology: Interesting finding
-
Received: ,
Accepted: ,
How to cite this article: Gupta S, Kumar P, Kumari N, Soni A. Cervical lymphadenopathy with dual pathology: Interesting finding. CytoJournal 2023;20:25.
A 48-year-old male presented with a rapidly enlarging painless firm to hard, mobile, and non-tender lymph node approximately 2 cm deep in the left level II cervical region. Fine needle aspiration of the lymph node was performed (Figure 1).
- (a) Cellular smear showing discrete population of round to polygonal cells with pyknotic nuclei and dense cytoplasm with smoothly curved filarial worm covered with a hyaline sheath and a pointed tail. (b) Higher magnification showing worm along with tumour cells with squamoid differentiation. (c) Cellular smear showing discrete population of tumor cells having squamoid appearance and of transverse section of gravid female worm with numerous ovoid eggs. (d) Higher magnification of smoothly curved worm covered with a hyaline sheath and a pointed tail, devoid of nuclei. (a) MGG ×10, (b) MGG ×40, (c) H&E ×10, (d) H&E ×40.
Q1. What is your interpretation?
Metastatic squamous cell carcinoma with microfilaria of Wuchereria bancrofti
Metastatic adenocarcinoma with microfilaria of W. bancrofti
Metastatic squamous cell carcinoma with microfilaria of Brugia malayi
Metastatic adenocarcinoma with microfilaria of B. malayi
Answer: a. Metastatic squamous cell carcinoma with microfilaria of Wuchereria bancrofti
The cytological findings are consistent with metastatic squamous cell carcinoma with accompanying microfilaria in a necro-haemorrhagic background. The filarial worm is smoothly curved, covered with a hyaline sheath and a pointed tail. The body has multiple discrete evenly spaced nuclei with no terminal nuclei in the tail. The microfilaria is identified as Wuchereria bancrofti owing to characteristic smoothly curved worm with pointed tail, devoid of nuclei (Figure 1)
Table 1 elaborates the key differentiating features of commonly occurring sheathed filarial nematodes.
| Nematode | Wuchereria bancrofti | Brugia malayi | Loa loa |
|---|---|---|---|
| Length | 244–296 µ | 177–230 µ | 231–250 µ |
| Cephalic space | Short | Long | Short |
| Tail | Pointed tail, devoid of nuclei | Two distinct nuclei on tail tip | Continuous discrete row of nuclei at the tail tip |
| Periodicity | Nocturnal | Nocturnal | Diurnal |
Q2. Which of the following is not a characteristic feature of W. bancrofti?
Pointed tail, devoid of nuclei
Two distinct nuclei on tail tip
Cephalic space is short
Length of microfilaria ranges from 244 to 296 µ.
Answer: b
Q3. What could be the possible reason for lodgement of filaria in the metastatic sites?
Increased vascularity of the tissues
Transmigration of microfilaria along with metastatic tumor emboli
Decreased host immune response
All of the above.
Answer: d
BRIEF REVIEW OF THE TOPIC
Filariasis is a vector borne public health issue and is endemic all over India. In India, W. bancrofti accounts for majority of the total filarial infections.[1] Filarial nematodes can be detected in peripheral blood, body fluids, urine, sputum, lymph node, thyroid, parotid, scrotum, and subcutaneous tissues along with a wide variety of metastatic sites.[2-4] Causative agents of human filariasis include W. bancrofti, B. malayi, Brugia timori, Loa loa, Mansonella perstans, Mansonella ozzardi, Mansonella streptocerca, and Onchocerca volvulus. Of all of the human filarial nematodes, W. bancrofti has the widest geographic distribution.[5] The existence of filarial nematodes in association with metastatic deposits could be coincidental or may involve a different pathogenetic mechanisms. Filarial parasites exert profound immunoregulatory effects on the host immune system with both parasite-antigen specific and more generalized levels of immune modulation.[6] The immune-modularity effects of microfilaria can be responsible for delayed post-operative healing after neck dissection.
Few authors have speculated that increased vascularity of the tissues and transmigration of microfilaria along with metastatic tumor emboli, decreased host immune response could be a possible reasons for lodgement of filarial in the metastatic sites.[3,7,8]
SUMMARY
This case highlights the importance of diligent screening of all cytology smears in endemic regions as the occurrence of microfilaria in cervical nodes of patients with oral cavity malignancy requiring neck dissection can have different implications in post-operative healing.
COMPETING INTEREST STATEMENT BY ALL AUTHORS
The authors declare that they have no competing interests.
AUTHORSHIP STATEMENT BY ALL AUTHORS
Each author has participated sufficiently in the work and takes public responsibility for appropriate portions of the content of this article. All authors read and approved the final manuscript. Each author acknowledges that this final version was read and approved.
ETHICS STATEMENT BY ALL AUTHORS
This study protocol was reviewed and approved by the Institutional ethics committee AIIMS, Raebareli. The approval number is F. 8/BIOETHICS/AIIMS-RBL/COMM/ OTH/2021/1 dated 03.07.2022.
LIST OF ABBREVIATIONS (In alphabetic order)
B. malayi – Brugia malayi
H&E – hematoxylin & eosin
MGG – May Grunwald Giemsa
W. bancrofti – Wuchcheria bancrofti
EDITORIAL/PEERREVIEW STATEMENT
To ensure the integrity and highest quality of CytoJournal publications, the review process of this manuscript was conducted under a double-blind model (the authors are blinded for reviewers and vice versa) through automatic online system.
References
- Epidemiology of communicable diseases In: Park's Textbook of Preventive and Social Medicine (26th ed). Jabalpur, India: Bhanot Publishers; 2021.
- [Google Scholar]
- Cytology of achylous hematuria: A clue to an underlying uncommon clinical scenario. Cytojournal. 2018;15:30.
- [CrossRef] [PubMed] [Google Scholar]
- Microfilaria in cytological smears at rare sites coexisting with unusual pathology: A series of seven cases. Trop Parasitol. 2012;2:61-3.
- [CrossRef] [PubMed] [Google Scholar]
- Microfilariae coexisting with a follicular lesion in thyroid aspirate smears in an uncommon case of a retrosternal thyroid mass, clinically presenting as malignancy. Cytojournal. 2011;8:4.
- [CrossRef] [PubMed] [Google Scholar]
- Diagnostic Identification and differentiation of microfilariae. J Clin Microbiol. 2019;57:e00706-19.
- [CrossRef] [PubMed] [Google Scholar]
- Immunology of lymphatic filariasis. Parasite Immunol. 2014;36:338-46.
- [CrossRef] [PubMed] [Google Scholar]
- Microfilaria in liver aspiration cytology: An extremely rare finding. Diagn Cytopathol. 2011;39:521-2.
- [CrossRef] [PubMed] [Google Scholar]
- Rare association of microfilaria with poorly differentiated mucin-secreting metastatic carcinoma in liver aspirate cytology. BMJ Case Rep. 2019;12:e231618.
- [CrossRef] [PubMed] [Google Scholar]
